BTK Knockout cell line(U-2932)

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BTK Knockout cell line(U-2932) Order

Catalog#

YKO-H067

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

BTK Knockout cell line (U-2932)

Morphology

Human Lymphocyte-like, suspension

Passage ratio

1：2-1：4

Species

Human

Gene Name

BTK

Gene ID

695

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10%FBS

Target region

Knockout region: Exon 2 (based on transcript BTK-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:U-2932			STR Info (Cell bank) Cell Line:U-2932
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	11	11		11	11
D7S820	9	13		9	13
D16S539	12	12		12	12
VWA	14	17		14	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	11	12		11	12
CSF1PO	10	12		10	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the BTK gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-2932 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, BTK gene knockout BTK Knockout cell line (U-2932) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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